Playing a sample across the keyboard

If chop a sample, lets say a one shot of a horn or a synth stab.
If play the one shot on my keyboard, is it safe to say that the key I play the sample on lets say I hit C# for example, that the sample would be played in that key, or would it just be played up or down the amount of semitones away from the samples original key?

This sounds confusing but please help?

I want to be able to just sample an instrument and then lets say play the FMaj Scale on my keyboard and use the sample as an instrument